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Quality control of supporting data within public databases is crucial to ensure accurate and the most up-to-date metadata however, quality control practices and methods are not readily known or clearly stated. However, since these downloads include thousands of complete bacterial genomes, there is a challenge to easily identify which genomes were included in the download, to determine if all files and metadata associated with particular genomes were included and whether supporting data were correct. The NCBI ftp site 7 provides links to download all bacterial genomes in a number of file types. Data can be readily downloaded from these databases through ftp sites or facilitated through download links. Additional databases with more specific microbial applications and bioinformatics programs include IMG ( Markowitz et al., 2012) and PATRIC (Pathosystems Resource Integration Center) ( Wattam et al., 2014). These whole bacterial genome sequence data are housed in publically available databases such as NCBI 4 ( Benson et al., 2015), European Molecular Biology Laboratory–European Bioinformatics Institute (EMBL–EBI) 5 ( Amid et al., 2012), and DNA Data Bank of Japan (DDBJ) 6 ( Kodama et al., 2012), which make up the International Nucleotide Sequence Database Collaboration (INSDC) ( Nakamura et al., 2013). As a result of advancements in sequencing technologies, with increased output and decreased costs, the number of completed genomes will continue to rise resulting in substantial amounts of data. Integrated Microbial Genomes (IMG) 2 ( Markowitz et al., 2012) reported the number of bacterial genomes at 26,033 at the finished (3,378), draft (1,683), and permanent draft (20,972) status, and there is a total of 39,969 bacterial genome sequencing projects listed in the Genomes OnLine Database (GOLD) 3 ( Reddy et al., 2015), an increase from only 1,986 in 2007. 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AutoCurE provides an automated approach to curate local genomic databases by flagging inconsistencies or errors by comparing the downloaded supporting data to the genome reports to verify genome name, RefSeq accession numbers, the presence of archaea, BioProject/UIDs, and sequence file descriptions. AutoCurE, an automated tool for bacterial genome database curation in Excel, was developed to facilitate local database curation of supporting data that accompany downloaded genomes from the National Center for Biotechnology Information. Publically available genomes can be readily downloaded however, there are challenges to verify the specific supporting data contained within the download and to identify errors and inconsistencies that may be present within the organizational data content and metadata. More than 3,000 bacterial genomes have been sequenced and are available at the finished status. 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It’s hard to think of a source of media that has had such a hold over what seems like the entire world. For Gen Xers, it was Friends and now, for Gen Z, it’s undoubtedly Euphoria. There always seems to be one show that each generation looks to for beauty and fashion inspiration. If you purchase an independently reviewed product or service through a link on our website, STYLECASTER may receive an affiliate commission. |
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